Journal: Frontiers in Physiology

Article Title: Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

doi: 10.3389/fphys.2023.1221310

Figure Lengend Snippet: iCre/Lox toolkit components available at Addgene.

Article Snippet: To further validate our material and iCre’s efficiency in transgenic experiments, we recombined 469-pME-iCre with p5E-UBI (5′clone containing the Ubiquitin promoter) into the optimized destination clone 1456-pDEST-miniTol2_R4R2_Cryst-eGFP (Addgene # 171795), and injected this final construct 114-UBI:iCre_crystGFP (Addgene #171797) with tol2 transposase to promote genomic integration ( ; ).

Techniques: Sequencing, In Vivo, Expressing, Plasmid Preparation, Construct, Clone Assay, Cloning, Marker, Control, Ubiquitin Proteomics

Journal: microPublication Biology

Article Title: Characterizing short germline-specific promoters with a range of expression levels in C. elegans

doi: 10.17912/micropub.biology.000843

Figure Lengend Snippet:

Article Snippet: We synthesized the destination plasmid as a clonal vector (Twist Bioscience, CA, USA) and included the appropriate acceptor BsaI sites, a consensus start site (aaaa), a gfp with two nuclear localization signals (SV40 and egl-13 ), a tbb-2 3’ UTR , and a universal MosTI backbone (pSEM246 ) that contains a non-rescuing cbr-unc-119 fragment and homology arms targeting a MosTI safe-harbor landing site on chromosome II .

Techniques: Plasmid Preparation